Auteur / Autrice : | Selma Čorovič |
Direction : | Lluis Maria Mir, Damijan Miklavčič |
Type : | Thèse de doctorat |
Discipline(s) : | Pharmacotechnie et physicochimie |
Date : | Soutenance en 2010 |
Etablissement(s) : | Paris 11 |
Partenaire(s) de recherche : | autre partenaire : Université de Paris-Sud. Faculté de pharmacie (Châtenay-Malabry, Hauts-de-Seine) |
Mots clés
Résumé
In vivo electroporation is used as an effective and safe tool for administration of a variety of extracellular agents such as chemotherapeutic drugs (e. G. Bleomycin), DNA or other molecules, which in normal conditions do not cross the cell membrane, into many different target tissue cells. The main focus of the present doctoral theses is the analysis of in vivo electroporation and corresponding local electric field distribution used in electrochemotherapy and electroporation based gene therapy and vaccination. The aims of this doctoral thesis cover three important issues in the development of electroporation based technologies and treatments: development of realistic numerical models of different tissues (i. E. Muscle, tumor and skin) and calculations and visualization of local electric field distribution in the models; validation of the realistic numerical models by in vivo experiments and development of a web-based interactive e-learning application on electroporation of cells and tissues and on electroporation based therapies and treatments. (e. G. Clinical electrochemotherapy of tumors and gene electrotransfer).