Interaction entre des facteurs de transcription et des ARN non codants et rôle de ces derniers dans la croissance et le développement racinaire d'Arabidopsis thaliana

par Andana Barrios

Projet de thèse en Biologie

Sous la direction de Martin Crespi et de Céline Sorin.

Thèses en préparation à Paris Saclay en cotutelle avec l'Universidad Nacional del Litoral , dans le cadre de Sciences du Végétal : du gène à l'écosystème , en partenariat avec Institut de Sciences des Plantes de Paris-Saclay (laboratoire) et de Université Paris-Sud (établissement de préparation de la thèse) depuis le 01-10-2018 .


  • Résumé

    The aim of this project is to understand how lncRNA may modulate the action of TFs, notably the NFY and/or APOLO-interacting TFs in order to reveal a potential novel regulatory mechanism of gene expression. These results will be also explored in tomato to translate this research on a crop. In the Argentinian lab, the PhD will characterize how specific TFs are able to recognize APOLO lncRNAs in vivo, and the impact of this interaction on their activity. In the French lab, the candidate will elucidate the mode of action of NF-YA2 and NF-YA10 in modulating root architecture as well as identified lncRNAs interacting with these TFs. Based on the knowledge obtained in both labs on these regulatory mechanisms, we will search for key orthologs in tomato of TF/lncRNAs interactors to set the basis for future studies on the conservation of their function in an interesting crop. The complementarity of these approaches will be maximized between labs, as the IPS2 lab has expertise in NF-YA TF biology, cell biology and RNA genomics whereas the IAL lab has strong skills in RNP biochemistry, lncRNA-chromatin interactions. Hence, regular exchanges of the PhD student through communication tools in addition to long-term stays are envisaged.

  • Titre traduit

    Interaction of transcription factors and non-coding RNAs in Arabidopsis root growth and development


  • Résumé

    The aim of this project is to understand how lncRNA may modulate the action of TFs, notably the NFY and/or APOLO-interacting TFs in order to reveal a potential novel regulatory mechanism of gene expression. These results will be also explored in tomato to translate this research on a crop. In the Argentinian lab, the PhD will characterize how specific TFs are able to recognize APOLO lncRNAs in vivo, and the impact of this interaction on their activity. In the French lab, the candidate will elucidate the mode of action of NF-YA2 and NF-YA10 in modulating root architecture as well as identified lncRNAs interacting with these TFs. Based on the knowledge obtained in both labs on these regulatory mechanisms, we will search for key orthologs in tomato of TF/lncRNAs interactors to set the basis for future studies on the conservation of their function in an interesting crop. The complementarity of these approaches will be maximized between labs, as the IPS2 lab has expertise in NF-YA TF biology, cell biology and RNA genomics whereas the IAL lab has strong skills in RNP biochemistry, lncRNA-chromatin interactions. Hence, regular exchanges of the PhD student through communication tools in addition to long-term stays are envisaged.