Structure-function relationship of biomimetic membranes with incorporated membrane proteins

par Sebastian KöHler

Projet de thèse en BIS - Biotechnologie, instrumentation, signal et imagerie pour la biologie, la médecine et l'environnement

Sous la direction de Donald Martin (edisce) et de Marco Maccarini.

Thèses en préparation à Grenoble Alpes , dans le cadre de École doctorale ingénierie pour la santé, la cognition, l'environnement (Grenoble) , en partenariat avec Techniques de L'Ingénierie Médicale et de la Complexité - Inofrmatique, Mathématiques et Applications. (laboratoire) et de SyNaBi : Systèmes Nanotechnologies, Biomimetiques et Biotechnologies (equipe de recherche) depuis le 16-02-2017 .


  • Résumé

    The nano-engineering of biomimetic membranes provides a sophisticated “self-assembled” approach to the manufacture of nanostructured devices. By incorporating membrane transport proteins in biomimetic membranes the group SyNaBi (within the laboratory TIMC-IMAG at Université Grenoble Alpes) showed that it is possible to built an artificial cell system for producing energy in a biomimetic fashion. Although that biomimetic energy device has reached quite a sophisticated stage of development, its further optimisation is currently limited by the lack of a complete understanding of the structure/function relationship of the system of the biomimetic membrane and incorporated transport proteins. The research proposed for this PhD thesis aims at overcomimg that limitation by utilising neutron reflectivity and electric impedance spectroscopy to characterise the molecular detail of the biomimetic membrane system and associate to its electrochemical behavior. The outcome of this PhD project will provide the results that would allow a great boost to the ongoing development of the biomimetic energy device, which would in-turn provide a strong basis for the miniaturisation and power for a raft of biomedical devices such as defibrillators, pacemakers that do not use leads, neurostimulators, muscle stimulators, implanted remote monitoring devices, or mechanical pumps such as to replace the urinary sphincter. The PhD will benefit from all the experimental techniques available in both ILL and TIMC-IMAG laboratories. In particular, Electric Impedance Spectroscopy (EIS) and Patch Clamp will be used to determine the electrochemical properties of the biomimetic membranes and the single channel conductivity of the porins, respectively. ElS will be use also in combination with Quartz Crystal Microbalance and Neutron reflectivity experiments. The latter will be of high importance to understand the structural properties of supported bilayers with inserted proteins and to indirectly determine the effect of proteins activation on the floating bilayer fluctuations. Reflectivity measurements will be carried out also at the European Synchrotron Radiation Facility in Grenoble.

  • Titre traduit

    Structure-function relationship of biomimetic membranes with incorporated membrane proteins


  • Résumé

    The nano-engineering of biomimetic membranes provides a sophisticated “self-assembled” approach to the manufacture of nanostructured devices. By incorporating membrane transport proteins in biomimetic membranes the group SyNaBi (within the laboratory TIMC-IMAG at Université Grenoble Alpes) showed that it is possible to built an artificial cell system for producing energy in a biomimetic fashion. Although that biomimetic energy device has reached quite a sophisticated stage of development, its further optimisation is currently limited by the lack of a complete understanding of the structure/function relationship of the system of the biomimetic membrane and incorporated transport proteins. The research proposed for this PhD thesis aims at overcomimg that limitation by utilising neutron reflectivity and electric impedance spectroscopy to characterise the molecular detail of the biomimetic membrane system and associate to its electrochemical behavior. The outcome of this PhD project will provide the results that would allow a great boost to the ongoing development of the biomimetic energy device, which would in-turn provide a strong basis for the miniaturisation and power for a raft of biomedical devices such as defibrillators, pacemakers that do not use leads, neurostimulators, muscle stimulators, implanted remote monitoring devices, or mechanical pumps such as to replace the urinary sphincter. The PhD will benefit from all the experimental techniques available in both ILL and TIMC-IMAG laboratories. In particular, Electric Impedance Spectroscopy (EIS) and Patch Clamp will be used to determine the electrochemical properties of the biomimetic membranes and the single channel conductivity of the porins, respectively. ElS will be use also in combination with Quartz Crystal Microbalance and Neutron reflectivity experiments. The latter will be of high importance to understand the structural properties of supported bilayers with inserted proteins and to indirectly determine the effect of proteins activation on the floating bilayer fluctuations. Reflectivity measurements will be carried out also at the European Synchrotron Radiation Facility in Grenoble.