Production, caractérisation et ingénierie de récepteurs lectines de type C : des outils dans la recherche d'immunomodulateurs.

par Silvia Achilli

Projet de thèse en Chimie Biologie

Sous la direction de Franck (csv) Fieschi et de Corinne Vivès.

Thèses en préparation à Grenoble Alpes , dans le cadre de Chimie et Sciences du Vivant , en partenariat avec Institut de Biologie Structurale (laboratoire) et de Laboratoire des Protéines Membranaires (LPM) (equipe de recherche) depuis le 01-06-2015 .

  • Titre traduit

    Production, characterization and engineering of C-­type lectin receptors as tools in immunomodulators research.

  • Résumé

    C-type Lectin Receptors (CLRs)are carbohydrate binding proteins expressed on the surface of myeloid cells. They interact with pahtogens and self antigens ligand through high affinity and high avidity interaction. These lectins offer tremendous potential to enhance the efficacy of vaccines and as therapeutic targets. A major objective would be to use CLRs as modulators in order to tailor the immune system response. To do so, molecules selective to each individual CLRs have to be developed. My research revolves around the development of recombinant production of 8 human C-type Lectin Receptors and the characterization of their ligands selectivity through the use of biophysical methods (SPR, ITC). Collaboration with the CIC-BiomaGUNE (Spain) for glycan-array analysis was established (two secondments). In the near future, structural characterization of the ligand binding mode will be conducted (X-Ray) to support, in strong interaction with chemists of the network, the rational improvement of new glycomimetics. The development of Lectin-based screening tools was conducted in collaboration with an industrial team (GLYcoDiag)