Development of biomimetic systems for the study of molecular motor oscillations

par Jonathan Lee Tin Wah

Thèse de doctorat en Biophysique

Sous la direction de Pascal Martin.

Le président du jury était Benoît Claude Forget.

Le jury était composé de Pascal Martin, Benoît Claude Forget, Andrea Parmeggiani, Didier Chatenay, Laurent Blanchoin, Jean Baudry.

Les rapporteurs étaient Andrea Parmeggiani, Didier Chatenay.

  • Titre traduit

    Développement de systèmes biomimétiques pour l'étude des oscillations des moteurs moléculaires

  • Résumé

    Pas de résumé en français

  • Résumé

    Recent studies have suggested that minimal actomyosin systems have the intrinsic property to oscillate whensubjected to an elastic load. A similar situation can be found in various biological systems, leading, both in-vivoand in-vitro, to spontaneous oscillations. In particular, muscular systems as well as mechanosensitive hair-cellbundles in the inner ear have been shown to oscillate spontaneously as the result of active force production by anacto-myosin protein complex. We attempt to shed light on the mechanism behind the oscillatory activity of theacto-myosin system, in particular by determining the parameters that control the frequency and amplitude ofoscillation. The stiffness of the system, the total force developed by the motors and the type of motors have beenproposed as being influential in this respect. To investigate this effect, we make use of a modified motility assayconsisting of a motor-driven stiff polarized actin bundle subjected to an elastic load provided by opticaltweezers. During the course of this work, we also characterized auto-assembled magnetic bead columns andassessed their viability as molecular force sensors to study the oscillations. The fact that they can easily beorganized into large arrays makes them interesting as potential ‘high-throughput’ force sensors

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