Thèse de doctorat en Médecine. Biologie parasitaire
Sous la direction de Jean-Michel Pinon.
Soutenue en 2004
à Reims .
Waterborne parasitic diseases are well identified, but they are few evaluated at present in France. Detection of main parasites, as Cryptosporidium oocysts and Giardia cysts, in water is well standardized. Because convergent problematic between oocysts of Cryptosporidium and Toxoplasma gondii we evaluate its implication in waterborne parasitic risks. After an experimental evaluation of risk, we describe the strategy to rapid detection of T. Gondii oocysts in water. We developed a methodology used for Cryptosporidium and Giardia detection: filtration of water samples, purification and elution of parasites, detection by bioassay and molecular biology. We then applied and validated this method in experimental and environmental conditions. We report our experience of 3 years survey in Champagne-Ardenne Region. Toxoplasmic DNA was present in 5% of samples. We purpose techniques assays for development of sentry detection method valuable in environmental conditions by sanitary authorities.
Waterborne parasitic risks and Toxoplasma Gondii incidence
Pas de résumé disponible.