Contribution à l'étude de l'assemblage des complexes respiratoires chez Saccharomyces cerevisiae

Contribution to the study of mitochondrial respiratory complex assembly in Saccharomyces cerevisiae

• Auteur / Autrice : Yann Saint-Georges Chaumet
• Direction : Geneviève Dujardin
• Type : Thèse de doctorat
• Discipline(s) : Génétique cellulaire et moléculaire
• Date : Soutenance en 2003
• Etablissement(s) : Versailles-St Quentin en Yvelines

Mots clés

Mots clés contrôlés

Saccharomyces cerevisiae

Interactions cardio-pulmonaires

Cytochrome c

Cytochrome-oxydase

Mitochondries

Résumé

The biognesis of the respiratory complexes in mitochondria is an intricate process that need the Oxa1p function. In S. Cerevisiae, the introduction of residu baring a positive charge in the transmembrane domain of two subunits of complex III : cytochrome c1 and Qcr9p, is able to compensate for the complex IV misassembly, due to the absence of the protein Oxa1p. The study of the mechanism of suppression suggest interaction between these positive charges and negative ones localised in the transmembrane domain of subunit of complex IV, allowing their insertion in the membrane. Oxa1p could allow the insertion of charged transmembrane domain in the mitochondrial inner membrane. The study of the suppressor mutations in presence of Oxa1p also shows interactions between subunits of complexes III and IV. Altogether these results suggest the presence of a supercomplexe III+IV and that the subunits of these complexes could interact before their insertion in the membrane